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Traditional PCR relies on thermal cycling (denaturation, annealing, extension) and requires specialized temperature control equipment, making it time-consuming. In contrast, isothermal amplification technologies (such as LAMP, RPA) can achieve exponential nucleic acid amplification at a constant temperature, providing results within 15-30 minutes, and are becoming a popular choice in fields like molecular diagnostics, pathogen detection, and rapid on-site testing.
The core "engine" of LAMP isothermal amplification —— Bst DNA polymerase, its performance is crucial to the experimental results!YINJIA BIOSCIENCES has dedicatedly developed and proudly launched a new generation of isothermal amplification core tool — Bst DNA Polymerase Large Fragment 2.0, to help you effortlessly master LAMP isothermal amplification experiments!
Product Description
Bst DNA Polymerase Large Fragment 2.0, is a high-performance DNA polymerase that has been genetically engineered and optimized for process efficiency. It perfectly retains the core activity required for isothermal amplification:
1.Powerful 5'→3' DNA polymerase activity, efficiently synthesizing new chains.
2.Excellent strand displacement activity, which can unwind double-stranded DNA without a denaturation step, is the key to achieving isothermal amplification!
3.Without 5'→3' exonuclease activity, effectively preventing the degradation of amplification products and ensuring the integrity and high yield of the reaction.More importantly, Bst 2.0 has achieved a significant surpass over the wild-type Bst in multiple key performance indicators!
Core Advantages
? Rapid amplification speed: Compared with the wild-type Bst, Bst 2.0 significantly improves the amplification speed and can complete the nucleic acid amplification cycle faster.
? Strong heat resistance:In the face of high-temperature reaction environments, Bst 2.0 can still function stably, and its enzyme activity is not affected by temperature fluctuations.
? Inhibitor-resistant: In terms of amplification speed, salt tolerance, heat resistance, and inhibitor tolerance, it outperforms the wild type and is very suitable for contamination-resistant isothermal amplification reactions.
? Batch Consistency: The production process follows the ISO 9001 quality management system to ensure consistency between production batches.
Application Fields
Rapid Detection of Pathogenic Microorganisms (Viruses, Bacteria, Fungi, etc.)
Point-of-Care Testing (POCT) and Bedside Diagnosis
Food Safety and Animal Disease Surveillance
Identification of Genetically Modified Components
Environmental Microbial Monitoring
Genotyping and SNP Detection
Isothermal Amplification Experiments in Basic Scientific Research
Order Information
Product Name | Bst DNA Polymerase Large Fragment 2.0 | |
Product Code | YJ-O-529 | |
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