Illumina Launches Novel 5-Base Sequencing Solution for Integrated Genomic and Epigenomic Analysis

On October 15, Illumina, Inc. announced the launch of a new 5-base solution for simultaneously detecting genomic variations and DNA methylation information in a single sample.

This solution adopts a new chemical conversion technology that can selectively convert methylated cytosine into thymine, preserving the genomic complexity and variation information of the sequencing library, thereby enabling the joint analysis of genomic and epigenetic information in a single sequencing run.

The solution includes two kits: Illumina 5-Base DNA Prep and Illumina 5-Base DNA Prep (Enrichment). The former supports genome-wide methylation detection, while the latter enables targeted enrichment analysis of specific genomic regions. Both products have completed early trials and are now officially available, offering single-base resolution for DNA methylation detection.

Library construction and sequencing workflows are compatible with Illumina's NovaSeq series and NextSeq™ 2000 sequencing platforms. The integrated DRAGEN algorithm can simultaneously perform methylation analysis and genomic variant detection within the same analytical pipeline.

This technology can be applied to disease mechanism research, detection of biomarkers associated with cancer and genetic diseases, disease progression analysis, and drug target research, providing new data support for precision medicine research. (Li Kewen, Interface News)