BioxYK Launches CelCode® High-Throughput Single-Cell Sequencing System Featuring High Flexibility and One-Click Automation
Strengthening the nation through advanced instrumentation is a crucial component of the strategy to build a leading scientific and technological power. Recently, China’s domestic single-cell sequencing capabilities have gained another “powerful tool.”
On the morning of March 29, 2024, Bestopcell (Beijing BaiAo YiKang Pharmaceutical Technology Co., Ltd., hereinafter referred to as “Bestopcell”) launched its new product, CelCode, at the 2nd International Single-Cell and Spatial Omics Conference held at the Guangming Cloud Valley International Conference Center in Shenzhen.®High-Throughput Single-Cell Sequencing System: Providing More Precise, Flexible, and User-Friendly Solutions for Diverse Sequencing Scenarios.
CelCode®The launch of the high-throughput single-cell sequencing system is a milestone, marking Bestopcell’s comprehensive transformation from product development to commercial sales and serving as a new starting point for the company’s commercialization journey. Furthermore, the release of this system signifies that Bestopcell has broken the foreign technological monopoly at its source, representing a significant milestone in advancing the commercialization of single-cell sequencing in China.
Liu Jie, founder of Bestopcell, stated: “CelCode®"The high-throughput single-cell sequencing system is a landmark achievement, boasting advantages such as high throughput, high detection efficiency, stable performance, and flexible intelligence. The launch of this system not only demonstrates bestopcell’s strength in scientific research and technological development but also brings revolutionary changes to life science research. We look forward to this system playing a significant role in future studies and making greater contributions to human health and the advancement of life sciences."
Liu Jie, Founder of Bestopcell
At the conference, Bestopcell was also honored with the "2023 Industry Milestone Event" award at the 2nd International Single-Cell and Spatial Omics Conference.
The CelCode released this time®High-Throughput Single-Cell Sequencing System is a single-cell sequencing technology platform independently developed by bestopcell. The system includes CelCode®Driver Instruments, CelCode®High-Throughput Single-Cell Transcriptome Kit, CelScene®Bioinformatics Analysis Software. This system leverages microfluidics, microdroplet encapsulation, and single-cell barcode labeling technologies to enable high-throughput cell capture, labeling, and sequencing detection.
Bestopcell CelCode®High-Throughput Single-Cell Sequencing System
CelCode®The system employs a flexible configuration of 1–4 chip channels, adjustable according to sample volume requirements. This eliminates the need for sample pooling, prevents chip wastage, and makes research more flexible, efficient, and cost-effective. The system offers high throughput, with four independently controlled channels capable of processing four separate samples simultaneously. Each sample can be configured to capture 500–10,000 single cells, with a multiplet rate of less than 0.7% (at 1,000 cells) and a cell capture efficiency exceeding 65%. The entire workflow—including water-in-oil emulsion generation, nucleic acid capture, and cell labeling—is completed in just 6 minutes.
CelCode®The system first uses microfluidic chips to encapsulate single cells, individual gel beads bearing barcodes and primers, and the reverse transcription reaction mixture within oil droplets. Reverse transcription is then performed to generate barcode-tagged cDNA. Subsequently, the cDNA is recovered for library preparation and high-throughput sequencing, enabling the simultaneous acquisition of gene expression data from thousands of single cells. Based on these data, a series of analyses can be conducted, including reconstruction of cell subpopulations and screening of markers between cell populations. Empirical data results demonstrate that CelCode®It demonstrates high stability in terms of captured cell count, median genes per cell, and reproducibility of cell clustering.
Bestopcell CelCode®Principles of Single-Cell Sequencing
CelCode®High-Throughput Single-Cell Sequencing System: Two Independent Tests on Human PBMC Samples
Compared to the past, which required cumbersome steps such as instrument and reagent preparation, sample storage, and sample processing, CelCode®High-throughput single-cell sequencing systems enable fully automated, one-click operations for high-throughput single-cell isolation, capture, and labeling.
Furthermore, Bestopcell has expanded its product line to cover the entire single-cell sequencing workflow, incorporating the critical upstream step of single-cell sample pre-processing, which is vital for ensuring result quality. Liu Jie told VCBeat that traditional single-cell sequencing requires fresh samples for testing immediately after collection due to limitations in storage technology, aiming to prevent tissue and cell inactivation. This requirement not only increases testing costs but also restricts the application of single-cell analysis in many scenarios. In contrast, using the pre-processing kits from Bestopcell’s product line enables researchers to conduct testing experiments even after tissues or cells have been cryopreserved for several months. This significantly enhances the flexibility of scheduling single-cell detection experiments, thereby avoiding waste of human, material, and financial resources.
“Bestopcell has achieved innovative advancements in single-cell sequencing technology, enabling faster and more stable execution of key technical processes—from sample processing, high-throughput single-cell isolation, and sequencing library construction to data analysis and clinical significance mining—thereby providing a full-chain solution for single-cell omics,” summarized Liu Jie.
It is reported that the equipment, systems, and raw materials of this platform are independently developed and manufactured by Bestopcell, with full independent intellectual property rights, ensuring safety and reliability. Currently, CelCode®High-throughput single-cell sequencing systems have been deployed at more than ten universities and research institutes. With its official debut, the system is poised to unlock broader application prospects.
Bestopcell High-Throughput Single-Cell Sequencing Service Process
Since its establishment, Bestopcell has served hundreds of clients. The innovation of its product system is a tangible embodiment of the team’s industry insights and distilled technical expertise. Upholding the philosophy that “technology creates value and serves human health,” Bestopcell will continue to strengthen its efforts in the field of single-cell detection, launching a series of related products. This will promote the application of single-cell detection across broader domains, ensuring that this revolutionary technology benefits more people.
Bestopcell, established in 2021, was co-founded by scientists from the Chinese Academy of Sciences, overseas-returnee scientists, clinical experts, and senior R&D specialists in high-throughput testing from renowned domestic institutions. The company is dedicated to providing cutting-edge, comprehensive solutions for single-cell and spatial omics.
Bestopcell’s current management and technical teams possess extensive experience in single-cell and spatial omics services and R&D. With nearly a decade of deep expertise in this field, the company has accumulated service experience involving over 150 different tissue types and more than 10,000 samples. Clients served by Bestopcell have published over 100 papers, with a cumulative impact factor exceeding 1,000, including publications in top-tier journals such as Cell, Nature, and Science (CNS).
Articles Supported by the bestopcell Technical Team (Partial List)
In addition to the newly launched products, the company also possesses the 10x Genomics single-cell platform, the Mission Bio single-cell platform, the 10x Genomics Visium CytAssist spatial transcriptomics platform, and the Olink protein detection platform. It provides a range of related technical services, including sequencing analysis at the single-cell level for transcriptomics, genomics, proteomics, and spatial omics, as well as molecular biology testing. The company also offers its independently developed reagent kits.
With the widespread adoption of precision medicine, high-throughput single-cell sequencing technology will see increasingly broad applications in oncology, neuroscience, immunology, microbiology, and other fields. Bestopcell and its CelCode®High-throughput single-cell sequencing systems will also usher in a new wave of incremental growth opportunities.