GMP-Grade NK Cell Expansion Kit Breakthrough: Over 20 Billion Cells Produced in 1L Culture System!

Natural Killer (NK) Cells: The Core Force of the Innate Immune System

Xingyiang BiotechiPSC-CAR-NK products,Beijing Jingda Biotechnology Co.,Ltd.NK Cell Injection,Hangzhou Qihan Biotech Co., Ltd.Universal CAR-NK andLegend BiotechThe pipelines laid out by giants such as Legend Biotech, Hangzhou Qihan Biotech Co., Ltd., Affimed, Fate Therapeutics, Nkarta, ImmunityBio, Xingyiang (Shanghai) Biotechnology Co., Ltd., and Beijing Jingda Biotechnology Co., Ltd., are all actively advancing in clinical research for hematologic malignancies (such as leukemia and lymphoma) and solid tumors (such as liver cancer and gastric cancer), with the aim of developing safer and more accessible "off-the-shelf" therapies.

NK Cell Therapy: A Promising Cancer Treatment with ChallengesThe application of bioreactors has met the demand for large-scale expansion of NK cells in a closed system, advancing the progress of standardized NK cell preparation and the launch of NK cell immunotherapy products.

ACROBiosystems has developed a high-performance culture kit for the in vitro expansion and cultivation of NK cells, based on a well-established cell culture platform, flow cytometry validation platform, and a strict quality management system:CelThera™ GMP NK Cell Activation and Expansion Kit (Phenol Red-free)(Can add phenol red supplement according to usage requirements), helping you overcome the challenge of NK cell expansion in vitro.

We used cryopreserved PBMCs (peripheral blood-derived) as seed cells, performed activation culture in T75 culture flasks, and transferred 2E7 viable cells to a 2L perfusion culture bag after 7 days for continuous perfusion culture expansion in a scalable rocking bioreactor WAVE.Ten days later, the cell density reached 2E7/mL, with over 20 billion cells harvested. At the time of harvest, the NK cell purity was above 95%, maintaining high killing efficiency against K562.

• Total Cell Expansion Performance and Cell Viability

PBMC cells were cultured with NK cell activation medium for one week and then transferred to a WAVE perfusion culture for 10 days. The cell expansion results showed that ACROBio's NK cell expansion medium could efficiently support cell growth while maintaining high cell viability.

• NKProportion of Positive Cells

PBMC cells were cultured with NK cell activation medium for one week and then transferred to a WAVE perfusion culture for 10 days. Flow cytometry results showed that ACROBio's NK cell culture medium could activate and maintain a high proportion of NK cells in culture, with a sustained high expression of CD16 positivity.

• NK Cell Activation Markers and Inhibition Markers

• NK Cell Cytotoxicity In Vitro

PBMC cells were cultured with NK cell activation medium for one week and then transferred to WAVE perfusion culture for 10 days. In vitro killing results showed that NK cells cultured with ACROBio's NK cell medium efficiently killed K562 cells, demonstrating the ability to effectively kill target cells in vitro.

CelThera™ GMP NK Cell Activation Culture Kit contains1L、2L、3L、4L、5Lin a variety of specifications. If interested, please scan the QR code below for inquiries and purchases!